Kodi Cryovial Imatanthawuza Chiyani Ngati "Si Yogwiritsidwa Ntchito Pagawo Lamadzi la Nayitrogeni Wamadzi"?

Mawu awa amafunsa funso: "Chabwino, ndi mtundu wanji wa vial wa cryogenic ngati sungagwiritsidwe ntchito mu nayitrogeni wamadzi?"
Sipadutsa sabata kuti tisafunsidwe kufotokoza chodzikanira chomwe chikuwoneka ngati chosamvetsetseka chomwe chimapezeka patsamba lililonse lazinthu zamtundu uliwonse mosasamala kanthu za wopanga, mosasamala kanthu za kuchuluka kwake komanso ngakhale ndi ulusi wamkati wa cryovial kapena ulusi wakunja wa cryovial.
Yankho ndilakuti: Iyi ndi nkhani yaudindo osati funso lokhudza mtundu wa cryovial.
Tiyeni tifotokoze.
Monga machubu ambiri olimba a labotale, ma cryovials amapangidwa kuchokera ku kutentha kokhazikika kwa polypropylene.
Kuchuluka kwa polypropylene kumatsimikizira kutentha kotetezeka.
Machubu ambiri a 15mL ndi 50mL amakhala ndi makoma owonda omwe amalepheretsa kugwiritsa ntchito kwawo kutentha kosachepera -86 mpaka -90 Celsius.
Makoma owonda amafotokozeranso chifukwa chake machubu a 15mL ndi 50mL samalangizidwa kuti azizungulira mwachangu kuposa 15,000xg popeza pulasitikiyo imatha kugawika ndikusweka ngati itagwiritsidwa ntchito mopitilira malire.
Mbale za cryogenic zimapangidwa kuchokera ku polypropylene yokhuthala yomwe imawalola kuti azitha kupirira kutentha kozizira kwambiri ndi kuwomba mu centrifuge pa liwiro lopitilira 25,000xg kapena kupitilira apo.
Vuto limakhala ndi kapu yosindikizira yomwe imagwiritsidwa ntchito kuteteza cryovial.
Kuti cryovial iteteze bwino minofu, selo kapena kachilombo ka HIV komwe kali, kapuyo iyenera kupukuta kwathunthu ndikupanga chisindikizo chosadukiza.
Mpata wawung'ono udzalola kuti madzi asungunuke ndi kuipitsidwa pachiwopsezo.
Khama lolimbikira limapangidwa ndi opanga ma cryovial kuti apange chosindikizira chapamwamba kwambiri chomwe chitha kukhala ndi silicon o-ring ndi/kapena ulusi wandiweyani womangira chipewacho pansi.
Uku ndiye kuchuluka kwa zomwe wopanga ma cryovial angapereke.
Pamapeto pake kupambana kapena kulephera kwa cryovial kusunga chitsanzo kugwa kwa katswiri wa labu kuti atsimikizire kuti chisindikizo chabwino chapangidwa.
Ngati chisindikizo sichikuyenda bwino, ndipo ngakhale ngati kapu yatsekedwa bwino, nayitrogeni yamadzimadzi imatha kulowa mu cryovial ikamizidwa mumadzi amadzimadzi a nitrogen.
Ngati chitsanzocho chasungunuka mofulumira kwambiri, nayitrogeni yamadzimadzi idzakula mofulumira ndikupangitsa kuti zomwe zili mkati mwake ziphulike ndikutumiza zidutswa zapulasitiki m'manja ndi kumaso kwa aliyense mwatsoka kukhala pafupi.
Chifukwa chake, kupatulapo kawirikawiri, opanga ma cryovial amafuna omwe amawagawa kuti awonetse molimba mtima chodzikanira kuti asagwiritse ntchito ma cryovials awo kupatula gawo la mpweya wa nayitrogeni wamadzi (kuzungulira -180 mpaka -186C).
Mutha kuwunikira mwachangu zomwe zili mu cryovial mwa kuzimiza pang'ono mu gawo lamadzi la nayitrogeni;ndi zolimba mokwanira ndipo sizingasweka.
Mukufuna kudziwa zambiri za kuopsa kosunga Mbale za cryogenic mumadzi amadzimadzi a nitrogen?
Nayi nkhani yochokera ku UCLA's Center for Laboratory Safety yolemba kuvulala chifukwa cha kuphulika kwa cryovial.